Increases in Retrograde Injury Signaling Complex-Related Transcripts in Central Axons following Injury

Figure 4

Importin 1 protein levels are significantly increased at 1 and 6 hours of injury. (a) Representative Western blots of several RIS-associated proteins (normalized to GAPDH). Note that GAPDH levels vary slightly across time points. Western blots also indicate exclusion of NeuN protein from axonal population. (b) Quantification of relative importin 1 protein expression. (c) Quantification of relative RanBP1 protein expression. Data are represented as mean ± SEM. Effect of time on expression was assessed using one-way repeated measures ANOVA, and mean values at each time point were compared to control post hoc using Dunnett’s test. indicates significant difference from control (p < 0.05); untransformed data are plotted; however, values were reciprocally transformed prior to ANOVA testing, to meet the requirement of normality. Transformed values are shown in Table 3.

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