Research Article

Mechanisms Underlying Adaptation of Respiratory Network Activity to Modulatory Stimuli in the Mouse Embryo

Figure 5

Embryonic respiratory pacemaker neurons are sensitive to DAMGO. (a) Schematic of an embryonic transverse slice showing location of a preBötC pacemaker neuron monitored with calcium imaging. (b) Calcium transients recorded from the cell in control conditions (top), after synaptic isolation (middle, cocktail), and in the presence of 0.25 μM DAMGO (bottom). After isolation, the cell’s rhythmic activity was suppressed in the presence of DAMGO. (c) Pooled data showing mean frequency (± SEM) of activity expressed by 20 pacemaker neurons in cocktail (white bar) and under the presence of DAMGO (black bar). Asterisk: .
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