The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In Vitro

Figure 1

Immunofluorescence imaging of SGN explants cultured with different concentrations of matrigel. (a) The diagram of the assay. (b) The schematic of dissection and culture of SGN explants from neonatal mice cochleae. The middle turn of P3 cochlea was dissected out, the stria vacularis, the organ of Corti, and modiolus were discarded, and remnant SGN bulk was cut into four equal pieces, attached to coverslips, and cultured. (c) Representative SGN explants stained with DAPI (blue) and anti-β-tubulin (green) antibody for each experimental condition.

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