The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In Vitro

Figure 6

3D culture improved the sprouting of neurites in SGN explants. (a) The diagram of the assay. (b) Representative images of SGNs explants cultured in 2D and 3D systems. DAPI, blue; β-tubulin, green. (c–f) Quantifications of the average number of primary dendrites, total branch length, number of branch tips, and number of branch points in 2D and 3D systems. , , and .

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