Table of Contents Author Guidelines Submit a Manuscript
Neural Plasticity
Volume 2016, Article ID 4783836, 13 pages
Research Article

Inhibition of DNA Methylation Impairs Synaptic Plasticity during an Early Time Window in Rats

1Department of Pathology and Physiology, School of Medicine, Faculty of Medicine, University of Valparaíso, 2341386 Valparaíso, Chile
2Interdisciplinary Center for Innovation in Health (CIIS), University of Valparaíso, 8380492 Valparaíso, Chile
3Institute of Physiology I, Systemic and Cellular Neuroscience, Albert-Ludwigs University Freiburg, 79104 Freiburg im Breisgau, Germany
4CINV-Universidad de Valparaíso, 2360102 Valparaíso, Chile
5Instituto de Biología, Pontificia Universidad Católica de Valparaíso, 3100000 Valparaíso, Chile

Received 4 April 2016; Revised 10 June 2016; Accepted 15 June 2016

Academic Editor: James M. Wyss

Copyright © 2016 Pablo Muñoz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Although the importance of DNA methylation-dependent gene expression to neuronal plasticity is well established, the dynamics of methylation and demethylation during the induction and expression of synaptic plasticity have not been explored. Here, we combined electrophysiological, pharmacological, molecular, and immunohistochemical approaches to examine the contribution of DNA methylation and the phosphorylation of Methyl-CpG-binding protein 2 (MeCP2) to synaptic plasticity. We found that, at twenty minutes after theta burst stimulation (TBS), the DNA methylation inhibitor 5-aza-2-deoxycytidine (5AZA) impaired hippocampal long-term potentiation (LTP). Surprisingly, after two hours of TBS, when LTP had become a transcription-dependent process, 5AZA treatment had no effect. By comparing these results to those in naive slices, we found that, at two hours after TBS, an intergenic region of the RLN gene was hypomethylated and that the phosphorylation of residue S80 of MeCP2 was decreased, while the phosphorylation of residue S421 was increased. As expected, 5AZA affected only the methylation of the RLN gene and exerted no effect on MeCP2 phosphorylation patterns. In summary, our data suggest that tetanic stimulation induces critical changes in synaptic plasticity that affects both DNA methylation and the phosphorylation of MeCP2. These data also suggest that early alterations in DNA methylation are sufficient to impair the full expression of LTP.