Inhibition of DNA Methylation Impairs Synaptic Plasticity during an Early Time Window in Rats

Figure 2

Late inhibition/capturing of DNMT has no effect on the transcription-dependent phase of LTP. (a) Less L-LTP was induced by TBS in slices treated with actinomycin-D (25 μM; open circles) than in slices treated with vehicle (0.01% DMSO; closed circles). Inset: representative traces are shown 2 min before (gray line) and 180 min after (black line) LTP induction. The calibration bar indicates 1 mV and 5 ms. (b) Hippocampal slices were exposed to a 5AZA or vehicle (gray bar) at 120 min after tetanization. The amount of L-LTP that was induced by TBS was not affected in the slices treated with 5AZA (25 μM; open circles) compared to the slices treated with vehicle (0.001% CH3COOH; closed circles). Inset: representative traces are shown before (gray line) and at 180 min after (black line) LTP induction. For all panels, the calibration bar indicates 1 mV and 5 ms; the error bars indicate the SE and numbers in parentheses correspond to the number of animals and number of slices.

(a)

(b)