Inhibition of DNA Methylation Impairs Synaptic Plasticity during an Early Time Window in Rats

Figure 5

The phosphorylation of MeCP2 at S80 at two hours after tetanic stimulation. (a) Representative immunostaining for phospho-S80 MeCP2 (red) and nuclear marker Hoechst 33342 (blue) in the hippocampal CA1 region in naïve (number of nuclei = 198) and tetanized (number of nuclei = 173) slices at two hours after TBS. Calibration bar, 50 μm. (b) Quantification of phospho-MeCP2 reactivity for data such as that shown in (a). , using Student’s -test. (c) Immunostaining for phospho-S80 MeCP2 (red) and the neuronal marker Hoechst 33342 (blue) in naïve slices incubated in the absence (left panel) or presence of treatment with an immunogen peptide (right panel) to which the relevant antibody was generated.

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