Dose-Dependent Differential Effect of Neurotrophic Factors on In Vitro and In Vivo Regeneration of Motor and Sensory Neurons

Figure 2

Representative images of RT97 stained neurites from spinal cord slices (a–c) and DRG neurons (d–f) cultured within a 3D collagen matrix alone (a, d) and with addition of 50 ng/mL of NGF (b, e) or NT-3 (c, f). Quantification of the number of neurites grown at increasing distance from the spinal cord slices (g, h) and from DRG body (k, l) after the addition of NGF and NT-3. Plots of the quantified AUC from (g), (h), (k), and (l) graphs for motor (i, j) or sensory (m, n) neurite outgrowth. Data expressed as mean ± SEM. versus control; . Scale bar: 200 μm (a–f).