Effects of Microtubule Stabilization by Epothilone B Depend on the Type and Age of Neurons

Figure 3

Effects of epothilone B on axon growth and cell viability in postnatal DRG neurons. (a–c) Postnatal day 4 (P4) DRG neurons were cultured in the absence or presence of various concentrations of epothilone B, from 1 pM to 1 μM, as indicated. Neurons were fixed at 24 hr after plating and immunostained with anti-Tuj1 antibodies. Representative images ((a), inverted images from Tuj1 immunostaining) and quantification of axon length (b) are shown. Cell viability was assayed by PI staining (c). Scale bar, 100 μm. < 0.05, < 0.01, and < 0.001; ns, statistically not significant.