Blockade of Toll-Like Receptors (TLR2, TLR4) Attenuates Pain and Potentiates Buprenorphine Analgesia in a Rat Neuropathic Pain Model

Figure 2

Quantitative real-time PCR analysis of CD40 (a, b), TLR2 (c, d), TLR4 (e, f), MyD88 (g, h), and TICAM2 (i, j) mRNA levels in the ipsilateral dorsal lumbar spinal cord (a, c, e, g, and i) and DRG (b, d, f, h, and j) tissue on 2nd, 7th, and 14th days after chronic constriction injury (CCI) in rats. The data are presented as the mean ± SEM, which represent normalized averages derived from the threshold cycles obtained in qRT-PCR from 6–8 samples per group. Intergroup differences were analyzed using one-way ANOVA followed by Bonferroni’s multiple comparisons test. , , and indicate significant differences versus the INTACT group.