Inhibition of the cAMP/PKA/CREB Pathway Contributes to the Analgesic Effects of Electroacupuncture in the Anterior Cingulate Cortex in a Rat Pain Memory Model

Figure 9

EA inhibited the colocalization of p-PKA with p-CREB in the ACC of pain memory model rats. The right ACC tissues from each groups were prepared and labeled with double-immunofluorescence (, five slides for each rat). (a) Views of the colocalization of p-PKA with p-CREB (scale bar, 20 μm). (b) The magnified image shows the colocalization of p-PKA with p-CREB. (c) Double-labeled immunofluorescence analysis demonstrated that EA significantly reduced the numbers of colocalized p-PKA and p-CREB. Error bars indicated SEM. and , versus the control group; , versus the model group; , versus the indo group; , versus the sham EA group.

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