Regeneration of Zebrafish CNS: Adult Neurogenesis

Figure 5

Proliferation and neurogenesis in zebrafish spinal cord. (a-b) Longitudinal sections of uninjured (a) and injured (b) cord showing Hu/BrdU colocalization around ependyma, indicating injury induced proliferation and neurogenesis. Star demarcates the injury epicenter; EPC: ependymal canal. (c) Violet boxed area in (b) represents Hu/BrdU colocalised cells (white arrowheads) in 7 DPI cord at higher magnification. (d) represents ultrastructure of a newly born neuron at the injury epicenter of an injured cord, with high nucleocytoplasmic ratio and very few organelles; the arrow points at the boundary of cytoplasm. (e) Blue boxed area in (b), showing subpial neuronal precursors stained with Hu/BrdU (grey arrowheads) in injured spinal cord at higher magnification. (f) An ultrastructural view of subpial neuron (black arrowhead) near the pial membrane (red arrow). (g) Heat maps representing genes related to neurogenesis differentially expressed during regeneration of zebrafish spinal cord. Scale bar = 200 μm (a, b), 5 μm (c, e), 2 μm (d), and 1 μm (f).