Drosophila Torsin Protein Regulates Motor Control and Stress Sensitivity and Forms a Complex with Fragile-X Mental Retardation Protein

Figure 1

The altered expression levels of Drosophila Torsin protein in transgenic flies. (a) The western blots of various strains expressing UAS-DTor-cDNA, pWiz- or UAS-DTor-RNAi, or both of UAS-DTor-cDNA and UAS-DTor-RNAi in all tissues using Tub-Gal4 drivers. UAS-DTor lines increased expression of DTor compared to that of genetic background (Tub-Gal4/+). Expressing two independent DTor-RNAi lines using Tub-Gal4 drivers induced significantly reduced DTor. At least 2 different sizes of DTor bands were detected from genetic backgrounds and flies expressing DTor-RNAi. However, 1 or 2 additional bands were detected from UAS-DTor overexpressing lines. The expression of the Drosophila homologue of Bip and heat shock 70-kDa cognate protein 70-3 (HSC70-3) was reduced in most transgenic flies compared with that of controls. (b) Quantification of the normalized amount of DTor in various strains. Two UAS-DTor-cDNA lines had significantly increased expression levels compared to those of Tub-Gal4/+. In contrast, the expression of DTor proteins was downregulated when two independent double-stranded RNA inhibitory constructs for DTor were expressed by Tub-Gal4 promoters. Simultaneous expression of both DTor-cDNA and DTor-RNAi constructs produced DTor expression levels approximately 1.5 times higher than those of genetic back ground (Tub-Gal4/+). (c) The expression of HSC70-3 (Bip) was significantly reduced in all transgenic fly, except one expressing pWIZ-DTor-RNAi. , , and .

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