Enhanced Neuroplasticity by the Metabolic Enhancer Piracetam Associated with Improved Mitochondrial Dynamics and Altered Permeability Transition Pore Function

Figure 5

Piracetam ameliorates mitochondrial fission induced by complex I inhibition. Rotenone induces mitochondrial fragmentation in (a) SH-SY5Y Ctl as well as (b) SH-SY5Y APPwt cells. This impairment can be reduced by piracetam (Pira). SH-SY5Y cells were seeded and incubated for one day. Afterwards, incubation was started with 1 mM piracetam (Pira) for 72 hours. During the last 24 hours, 2.5 µM rotenone was added. Afterwards, cells were stained and fixed for analysis of mitochondrial dynamics. ((c), (d)) Representative pictures of SH-SY5Y Ctl and ((e), (f)) SH-SY5Y APPwt cells treated with rotenone and/or piracetam (Pira); scale bar represents 10 µm. Data are represented as mean ± SEM; Two-Way ANOVA with Bonferroni’s posttest (, , and compared to rotenone treated cells); .

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