Research Article

The Habituation/Cross-Habituation Test Revisited: Guidance from Sniffing and Video Tracking

Figure 6

Sniff rate quartiles discriminate S1 proximity and consistently reveal WT mouse velocity. We explored how behavior varied as a function of sniff rate. For each trial type (PE3, S3) the 100 ms time bins were assigned to one of four quartiles of WT () sniff rates (0th–25th, 25th–50th, 50th–75th, and 75th–100th percentile, (a)). Their associated nose distance (b) and head angle (c) to the odor source, as well as their velocity (d), were determined. Distance and to some extent head angle to the object were lower for the S1 trial during the high sniff rate top quartile time bins. Velocity consistently increased with increasing sniff rates across all trial types. FUS mice showed similar patterns (not shown).