Table of Contents Author Guidelines Submit a Manuscript
Neural Plasticity
Volume 2016, Article ID 9802086, 11 pages
http://dx.doi.org/10.1155/2016/9802086
Research Article

Aromatase Expression in the Hippocampus of AD Patients and 5xFAD Mice

1Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
2Institute of Neuroanatomy, University Medical Center Hamburg Eppendorf, Martinistraße 52, 20246 Hamburg, Germany
3Institute of Neuropathology, University Medical Center Hamburg Eppendorf, Martinistraße 52, 20246 Hamburg, Germany

Received 9 December 2015; Revised 26 March 2016; Accepted 5 April 2016

Academic Editor: Ayanabha Chakraborti

Copyright © 2016 Janine Prange-Kiel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Table S1 provides detailed information (e.g. sex, age, postmortem time, etc.) about the human tissue samples that were used in this study. Sample pairs 1-10 were used for immunohistochemical analysis; samples 11-18 were used for PCR analysis.

Table S2 lists the sequences of the first exon-specific primers that were used to detect various variants of aromatase mRNA in human tissue. The table also provides the specific annealing temperatures for each primer pair and the length of the resulting PCR product.

Table S3 lists the sequences of the first exon-specific primers that were used to detect various variants of aromatase mRNA in mouse tissue. The table also provides the specific annealing temperatures for each primer pair and the length of the resulting PCR product.

Figure S1 Co-immunostaining for the astrocyte marker glial fibrillary acidic protein (GFAP) and aromatase revealed that most astrocytes in the human hippocampus did express aromatase at very low levels or not at all.

  1. Supplementary Material