Research Article

Pathological Role of Peptidyl-Prolyl Isomerase Pin1 in the Disruption of Synaptic Plasticity in Alzheimer’s Disease

Figure 3

Pin1 proteins show pathological loss in the synapses of human AD and Tg2576 AD model mice. The Pin1 proteins in synaptosomes, soluble synaptosomes, dendritic rafts, and PSD fractions were isolated from human frontal cortical tissues (a and b) and from Tg2576 AD model mice cortical tissues (c and d) and were detected by Western blot. (a and c) Representative Western blot experiment. (b and d) Results from densitometric imaging of these same samples (human AD frontal cortical tissues, ; human frontal cortical tissues, ; Tg2576 mice, ; and wild-type mice, ; , ). Ctrl: human control frontal cortical tissues; AD: human AD frontal cortical tissues; tSyn: the supernatant of synaptosomes extracted by 1% SDS; sSyn: the supernatant of synaptosomes extracted by 0.5% Triton X-100.