Mechanisms and Consequences of Dopamine Depletion-Induced Attenuation of the Spinophilin/Neurofilament Medium Interaction

<div>PKA overexpression is required for the spinophilin/NF-M interaction. (a) HEK293 cells were transfected with HA-tagged spinophilin, V5-tagged NF-M, and/or Myc-tagged PKAc. HA (goat antibody) immunoprecipitates were immunoblotted for HA (rabbit antibody), NF-M (mouse antibody), and Myc (mouse antibody). Data are representative of 3 independent experiments. (b, c) HEK293 cells were transfected with HA-tagged spinophilin, V5-tagged NF-M, and/or wild type or kinase dead (K72H mutant (MT)) myc-tagged PKAc. HA (goat antibody) (b) or V5 (rabbit antibody) (d) immunoprecipitates were immunoblotted for HA (rabbit antibody), NF-M (mouse antibody), and/or Myc (mouse antibody). 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Neural Plasticity

fig5

Figure 5

Figure 5: Mechanisms and Consequences of Dopamine Depletion-Induced Attenuation of the Spinophilin/Neurofilament Medium Interaction 