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Neural Plasticity
Volume 2017 (2017), Article ID 5959182, 11 pages
https://doi.org/10.1155/2017/5959182
Research Article

Hippocampal Regulation of Postsynaptic Density Homer1 by Associative Learning

1Neuroscience and Mental Health Research Institute, Cardiff University, Cardiff CF24 4HQ, UK
2MRC Centre for Neuropsychiatric Genetics and Genomics, Institute of Psychological Medicine and Clinical Neurosciences, Cardiff University, Cardiff CF24 4HQ, UK
3School of Biosciences, Cardiff University, Cardiff CF24 4HQ, UK

Correspondence should be addressed to Nicholas E. Clifton; ku.ca.ffidrac@ennotfilc

Received 14 July 2017; Revised 18 September 2017; Accepted 10 October 2017; Published 7 November 2017

Academic Editor: Anthony J. Baucum II

Copyright © 2017 Nicholas E. Clifton et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Genes involved in synaptic plasticity, particularly genes encoding postsynaptic density proteins, have been recurrently linked to psychiatric disorders including schizophrenia and autism. Postsynaptic density Homer1 proteins contribute to synaptic plasticity through the competing actions of short and long isoforms. The activity-induced expression of short Homer1 isoforms, Homer1a and Ania-3, is thought to be related to processes of learning and memory. However, the precise regulation of Homer1a and Ania-3 with different components of learning has not been investigated. Here, we used in situ hybridization to quantify short and long Homer1 expression in the hippocampus following consolidation, retrieval, and extinction of associative fear memory, using contextual fear conditioning in rats. Homer1a and Ania-3, but not long Homer1, were regulated by contextual fear learning or novelty detection, although their precise patterns of expression in hippocampal subregions were dependent on the isoform. We also show for the first time that the two short Homer1 isoforms are regulated after the retrieval and extinction of contextual fear memory, albeit with distinct temporal and spatial profiles. These findings support a role of activity-induced Homer1 isoforms in learning and memory processes in discrete hippocampal subregions and suggest that Homer1a and Ania-3 may play separable roles in synaptic plasticity.