Evidence of Presynaptic Localization and Function of the c-Jun N-Terminal Kinase

<div>JNK is localized in the presynaptic compartment. (a) Immunofluorescence on mouse cortical purified synaptosomes stained for p-JNK (green) and for Syntaxin-2 (red) colocalization is visualized as yellow signal in Merge image, with Pearson’s coefficient 0.54 ± 0.08. Images acquired with super resolution microscopy; scale bar 1 <i>μ</i>m. (b) Western blotting analysis of JNK and p-JNK active form presence in 20 <i>μ</i>g of total cortical lysates, postsynaptic density region (represented by PSD95 postsynaptic specific protein on TIF fraction) and presynaptic compartment (represented by Synaptophysin presynaptic specific protein on purified synaptosomes). JNK is localized both in postsynaptic and in presynaptic region and its phosphorylation state is comparable. Actin was used as loading control.</div>

Neural Plasticity

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Figure 1

Figure 1: Evidence of Presynaptic Localization and Function of the c-Jun N-Terminal Kinase 