Electrophysiological, Morphological, and Ultrastructural Features of the Injured Spinal Cord Tissue after Transplantation of Human Umbilical Cord Blood Mononuclear Cells Genetically Modified with the VEGF and GDNF Genes

Figure 5

Immunoelectron microscopic results. (А–D), (K–N) Immunohistochemical reaction in the area of spinal cord injury in hUCB-MCs + Ad5-VEGF + Ad5-GDNF group. (A) GFAP-positive astrocyte with narrow processes which limit small posttraumatic cavity (asterisk). (B) GFAP-positive fibrous astrocyte with numerous branched processes (arrows), which surround the cavity (asterisks) and form myelinated and unmyelinated fibers. (С) Cytoplasm of GFAP-positive fibrous astrocyte. Colloidal gold particles sit exclusively on intermediate filaments in the cytoplasm of cells (arrows). (D) GFAP-positive reaction (arrows) around the capillaries. (K) HNA-positive reaction in microglia nucleus. (L) HNA-positive cells with round nucleus and narrow rim of cytoplasm. (M) HNA-positive reaction in fibrous astrocyte nucleus. (N) HNA-positive reaction in elongated cell nucleus in the area of injury. (E–J) Immunohistochemical reaction in the area of spinal cord injury in hUCB-MCs group. (Е) GFAP-positive fibrous astrocyte. (F) GFAP-positive intermediate filaments in fibrous astrocyte cytoplasm. (G) GFAP-positive narrow astrocyte with thin processes. (H) HNA-positive reaction in microglia nucleus. (I) HNA-positive elongated cell with oval nucleus and oval cells with a round nucleus. (J) HNA-positive endothelial cell. Scale bar: 5 μм (A, D, G, J), 1 μм (B, N, E, I), 200 nm (C), 500 nm (K), 300 nm (L, F), 700 nm (M), and 800 nm (H). n: nucleus; mf: myelin fiber; m: mitochondria; en: endothelial cell; ScC: Schwann cell.