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Neural Plasticity
Volume 2018 (2018), Article ID 2062346, 10 pages
https://doi.org/10.1155/2018/2062346
Research Article

Identification of Binding Partners of Deafness-Related Protein PDZD7

1Shenzhen Research Institute of Shandong University, Shenzhen, Guangdong 518057, China
2Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, China
3Co-Innovation Center of Cell Biology, Shandong Normal University, Jinan, Shandong 250014, China

Correspondence should be addressed to Zhigang Xu; nc.ude.uds@gzux and Yanfei Wang; nc.ude.uds@fy_gnaw

Received 2 December 2017; Revised 24 January 2018; Accepted 14 February 2018; Published 28 March 2018

Academic Editor: Renjie Chai

Copyright © 2018 Haibo Du et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

PDZD7 is an important deafness gene, whose mutations are associated with syndromic and nonsyndromic hearing loss. PDZD7 contains multiple PDZ domains that are essential for organizing various proteins into protein complex. Several PDZD7-binding proteins have been identified, including usherin, ADGRV1, whirlin, harmonin, SANS, and MYO7A, all belonging to USH proteins. Here, we report the identification of novel PDZD7-binding partners through yeast two-hybrid screening using the first two PDZ domains of PDZD7 as bait. Eleven proteins were identified, most of which have not been reported as PDZD7-binding partners before. Among the identified proteins, ADGRV1, gelsolin, and β-catenin have been shown to play important roles in hearing, whereas the functions of other proteins in the inner ear remain elusive. We confirmed the expression of one candidate PDZD7-binding protein, CADM1, in the mouse inner ear and evaluated the auditory function of Cadm1 knockout mice by performing auditory brainstem response (ABR) measurement. Unexpectedly, Cadm1 knockout mice show normal hearing threshold, which might be explained by the possible compensation by its homologs that are also expressed in the inner ear. Taken together, our work identified several novel PDZD7-binding proteins, which will help us to further understand the role of PDZD7 in hearing transduction.