Identification of Binding Partners of Deafness-Related Protein PDZD7

Figure 1

Verification of protein interactions by performing yeast two-hybrid experiments. AH109 yeast cells containing either Gal4 BD/Gal4 AD, Gal4 BD-PDZD7/Gal4 AD, Gal4 BD/Gal4 AD-β-catenin, Gal4 BD/Gal4 AD-AMOT, Gal4 BD/Gal4 AD-CADM1, Gal4 BD-PDZD7/Gal4 AD-β-catenin, Gal4 BD-PDZD7/Gal4 AD-AMOT, or Gal4 BD-PDZD7/Gal4 AD-CADM1 were plated on nonselective (−Leu/Trp) and selective (−Leu/Trp/His) plates with the presence of 2.5 mM 3-AT and incubated at 30°C for 3 days.

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