Consumption of a High-Fat Diet Alters Perineuronal Nets in the Prefrontal Cortex

Figure 3

Consumption of a high-fat diet does not alter PNN intensity nor PNN number in the infralimbic prefrontal cortex (IL-PFC). (a) Schematic of the region analyzed. (b) Normalized PNN intensity expressed as a cumulative probability for chow (dashed line), HF-CM (gray line), and HF-AL (black line) groups. (c) Normalized PNN intensity expressed as a frequency distribution for chow, HF-CM, and HF-AL groups. (d) Normalized average PNN intensity among chow (white), HF-CM (gray), and HF-AL (black) groups. (e) PNN number, measured by the number of WFA+ cells. (f) Cell density, measured by the number of DAPI+ cells. (g) Representative images shown as max projections of z-stacks. Values represent the mean ± SEM (). indicates significant difference between HF-CM and chow as well as between HF-AL and chow.

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