Intramuscular Injection of Adenoassociated Virus Encoding Human Neurotrophic Factor 3 and Exercise Intervention Contribute to Reduce Spasms after Spinal Cord Injury

Figure 7

Compared with the sham surgery-treated group, the size of cholinergic interneurons in the central spinal cord gray matter (Rexed’s lamina VII) was smaller but the number did not change in each experimental group. (a) Schematic diagram of the spinal layer, with the white rectangular box indicating the position of the VII layer. Representative immunofluorescence staining of cholinergic interneuron ChAT (red) in each group. Scale bar: 20 μm. (b) Bar graph showing changes in the number of cholinergic interneurons in each group. Number of cells counted per field (123 μm × 89 μm). (c) Bar graph showing changes in the size of cholinergic interneurons in each group. Data are expressed as the mean ± S.E.M. One-way ANOVA was used, and each experimental group was compared with the control group. , . ns: nonsignificant.

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