Regulation of Neural Stem Cell Proliferation and Differentiation by Graphene-Based Biomaterials

Figure 5

Regulation of the proliferation and differentiation of NSCs on 3D graphene-based nanomaterials. (a) The proliferation of NSCs cultured on 3D graphene foam: (A) low-magnification SEM images of NSCs cultured on 3D graphene foams; (B) high-magnification SEM images of NSCs cultured on 3D graphene foam; (C) cell viability assay of NSCs cultured on 3D graphene foam; (D) immunofluorescence staining for nestin in NSCs cultured on 3D graphene foam after culturing for 5 days; (E) immunofluorescence staining of Ki67 and nestin in NSCs cultured on 3D graphene foam; (F) western blot analysis of Ki67 expression on 2D graphene films and 3D graphene foams. (b) The differentiation of NSCs on 3D graphene foam. (c) The differentiation of NSCs on stiff and soft graphene foam scaffolds.

(a)

(b)

(c)