Memantine Differentially Regulates Tau Phosphorylation Induced by Chronic Restraint Stress of Varying Duration in Mice

Figure 2

Memantine reduces tau hyperphosphorylation induced by 16 consecutive days of chronic restraint stress (CRS) in the mouse frontal cortex. (a) Frontal cortex extracts were analyzed by Western blotting with the AT8 and PS396 antibodies. Memantine reduced CRS-induced tau phosphorylation at the AT8 site, but not at the Ser396 site. CRS with or without memantine treatment had no statistically significant effect on the expression of total tau (TAU5). (b) Representative areas of AT8-positive immunofluorescent staining in cortices from the four groups of mice. (c–e) Densitometric quantification of the bands in (a). (f) Mice without CRS were treated with memantine in doses of 5 and 10 mg/kg for 16 days; tau phosphorylation and expression were analyzed by Western blotting. (g, h) Densitometric quantification of the bands in (f). The results are shown as means ± SEM ( in each group). GAPDH was used as a loading control. ; . Con: control.

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