Memantine Differentially Regulates Tau Phosphorylation Induced by Chronic Restraint Stress of Varying Duration in Mice

Figure 5

Memantine enhances tau phosphorylation after 28-day chronic restraint stress (CRS) in the mouse frontal cortex. (a) Results of Western blotting showed that memantine enhances tau phosphorylation at the AT8 site for both doses. Furthermore, the expression of the 3R tau isoform (RD3), 4R tau isoform (RD4), P-tau-PS396, and TAU5 was also detected among the four groups of mice. (b) Densitometric quantification of the bands in (a). (c) Representative areas of AT8-positive immunofluorescent staining in cortices from the four groups of mice. (d) Western blotting analyzed the effects of memantine administration with doses of 5 and 10 mg/kg for 28 days on tau phosphorylation and expression in frontal cortex of mice without CRS. (e, f) Densitometric quantification of the bands in (d). The results are shown as means ± SEM ( in each group). GAPDH was used as a loading control. ; ; . Con: control.

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