Changes in the Fluorescence Tracking of NaV1.6 Protein Expression in a BTBR T+Itpr3tf/J Autistic Mouse Model

Figure 1

Altered NaV1.6 expression and distribution in the PFC of the BTBR mouse model. (a, b) The red channel represents confocal images of NaV1.6 (antisodium channel), the blue channel represents ankyrin-G (Ank-G), and the green channel represents NeuN immunofluorescence in the PFC of C57BL/6J and BTBR mice. (c) The mask ROI was used to detect NaV1.6 expression within the NeuN soma. (d) The quantification of NaV1.6 immunofluorescence expression within the soma of C57BL/6J and BTBR mice. mice per group. (e) Representative confocal images of NaV1.6 (red) at the AIS in C57BL/6J and BTBR mice. (f) AIS tracking analysis of NaV1.6 in C57BL/6J and BTBR mice. mice per group. (g) NaV1.6 protein levels in PFC lysates from C57BL/6J and BTBR mice and the Western blot bands. The protein expression was normalized with β-actin. -5 mice per group. Data represent ; statistical differences were assessed using Student’s -test (). Scale bars represent 20 μm in (b) and 10 μm in the white box (zoom images in (b)) and (e).