Loss of Cochlear Ribbon Synapse Is a Critical Contributor to Chronic Salicylate Sodium Treatment-Induced Tinnitus without Change Hearing Threshold
Quantitative analysis of cochlear ribbon synapses in the SS-treated and control groups. (a) Overview image of the whole mounts staining included apical, middle, and basal turns. Cochlear ribbon synapses were labeled using anti-CtBP2 staining (red, below inner HCs); cell nuclei were stained with DAPI (blue). CtBP2 was also present in the nucleus. . (b) Enlarged images of apical, middle, and basal turns in panels of (a) (white frames); CtBP2 staining puncta were counted in randomly chosen regions containing four inner HCs. SS significantly reduced CtBP2 puncta numbers. . (c) Quantitative analysis of cochlear ribbon synapses in the SS and control groups. The average number of CtBP2 staining puncta in apical, middle, and basal turns was analyzed, respectively. Total numbers of CtBP2 staining puncta in the two groups were also analyzed. There was a significant loss of CtBP2 staining puncta in SS-exposed rats compared to the control group (all ).
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