Hsp70/Bmi1-FoxO1-SOD Signaling Pathway Contributes to the Protective Effect of Sound Conditioning against Acute Acoustic Trauma in a Rat Model

Figure 6

SOD1 and SOD2 protein expressions of SGNs after sound conditioning and acute noise exposure. Distributions and expressions of SOD1 (a) and SOD2 (b) protein were detected with the immune-fluorescence assay in the Ctrl and SC groups as well as the NE and SC+NE groups (A), while the quantity of these protein expressions was analyzed by fluorescence intensity (D). SOD1/SOD2 (red) and tubulin (green) as SGN marker were, respectively, labeled with fluorescent secondary antibody, and nuclei (blue) were labeled with DAPI. Scale bars represent 100 μm. Protein expressions of these were also assessed with western blot (B), while the quantity of these protein expressions was analyzed by integrated light density (C). β-Actin protein was available as an endogenous control. Furthermore, values are ( animals/group). vs. control group; vs. control group. Statistical analysis of the results presented in (C) was performed with one-way ANOVA (SOD1: , ; SOD2: , ), followed by Newman-Keuls’ post hoc test (, ; animals/group). Statistical analysis of the results presented in (D) was performed with one-way ANOVA (SOD1: , ; SOD2: , ), followed by Newman-Keuls’ post hoc test (, ; pictures from 3 animals/group). Furthermore, values are .

(a)

(b)