GPER-Deficient Rats Exhibit Lower Serum Corticosterone Level and Increased Anxiety-Like Behavior

Figure 3

Distribution of GPER along the HPA axis in the mouse. (a) GPER immunofluorescence (green) in the PVN; (b) presence of Tomato⁺ cells (red) in the PVN of GPER reporter (GPER Cre/tdTomato) mice; (c) immunohistochemistry shows Tomato⁺ (red) cells express astrocyte marker S100 (green) in the PVN of GPER reporter mice. Note that only a fraction of S100⁺ cells are Tomato⁺. (d) GPER RNAscope in situ hybridization signal (green) in mouse PVN. (e) Presence of Tomato⁺ (red) cells in the intermediate lobe (IL) and anterior lobe (AL) but not in the posterior lobe (PL) of the pituitary. (f) GPER RNAscope signal (green) in the pituitary of GPER reporter mice. Note the presence of clustered RNAscope signal (green) in Tomato⁺ (red) cells in the intermediate lobe and weak sporadic RNAscope signal in the anterior lobe, but the absence of RNAscope signal in the posterior lobe. (g) Presence of Tomato⁺ cells in the adrenal gland. Note that most cells in the adrenal medulla are Tomato⁺ (red). Some cells in the adrenal cortex, particularly in the zona fasciculata, are Tomato⁺. (h) Presence of Tomato⁺ cells in the superior cervical sympathetic ganglion of GPER reporter mice. (i) GPER RNAscope signal in the superior cervical sympathetic ganglion of WT mice. .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)