GPER-Deficient Rats Exhibit Lower Serum Corticosterone Level and Increased Anxiety-Like Behavior

Figure 7

GPER-deficient rats had lower basal serum corticosterone level. (a) Basal serum corticosterone level (detected by HPLC-MS) in WT and GPER^-/- rats of either sex. Note that serum corticosterone of GPER^-/- female rats () is significantly lower than that of WT female rats (). GPER^-/- male rats () also seemed to have lower serum corticosterone level than WT males (), but the difference did not reach statistical significance. (b) Basal serum17β-estradiol level (detected by HPLC-MS) in WT and GPER^-/- rats of either sex. Note that serum 17β-estradiol of GPER^-/- female rats () is significantly lower than that of WT female rats (). (c) Basal serum adrenaline level (detected by HPLC-MS) in WT and GPER^-/- rats of either sex. Note that serum adrenaline of GPER^-/- female rats () is significantly higher than that of WT female rats (). (d) Basal serum corticosterone level in WT and GPER^-/- rats of different menstrual cycle. (e) Basal serum 17β-estradiol level in WT and GPER^-/- rats of different menstrual cycle. (f) Basal plasma CRH level (detected by ELISA) in WT and GPER^-/- rats of either sex. Note that plasma CRH of GPER^-/- female rats () is significantly higher than that of WT female rats (). (g) Basal plasma ACTH level (detected by ELISA) in WT and GPER^-/- rats of either sex. , , , and , two-way ANOVA with Tukey post hoc test.

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