Predisposition of Neonatal Maternal Separation to Visceral Hypersensitivity via Downregulation of Small-Conductance Calcium-Activated Potassium Channel Subtype 2 (SK2) in Mice
Effects of the SK2 channel blocker apamin on , spontaneous neuronal firing rates, SK2 protein in membrane fraction, and pain threshold. (a, b) Apamin injection decreased the amplitude of in naïve mice (-test, , , neurons/5 mice per group). (c, d) Apamin injection increased the neuronal firing rates in the spinal DH of naïve mice (-test, , , neurons/5 mice per group). (e) Apamin injection resulted in the decrease of the spinal SK2 channel in membrane fraction in the naïve mice (-test, , , per group). (f) Apamin administration caused the decrease of the pain threshold in naïve mice (-test, , , per group). (g) Naïve mice with intrathecal injection of apamin exhibited increased AWR score in response to the distension pressure at 60 mmHg (Bonferroni post hoc test, ) (two-way ANOVA test, , , per group). Data are expressed as ; and .