Predisposition of Neonatal Maternal Separation to Visceral Hypersensitivity via Downregulation of Small-Conductance Calcium-Activated Potassium Channel Subtype 2 (SK2) in Mice
Effects of SK2 activator 1-EBIO on , spontaneous neuronal firing rates, SK2 protein in membrane fraction, and visceral pain threshold. (a, b) 1-EBIO administration increased the amplitude of in mice with neonatal MS (-test, , , neurons/5 mice per group). (c, d) 1-EBIO administration decreased the neuronal firing rates in the spinal DH of mice with neonatal MS (-test, , , neurons/5 mice per group). (e) 1-EBIO administration caused the increase of the spinal SK2 channel in membrane fraction in MS mice (-test, , , per group). (f) 1-EBIO administration effectively reversed the decrease of visceral pain threshold due to neonatal MS (-test, , , per group). (g) MS mice with intrathecal injection of 1-EBIO exhibited decreased AWR scores in response to the distension pressures at 40 mmHg (Bonferroni post hoc test, ) and 60 mmHg (Bonferroni post hoc test, ) (two-way ANOVA test, , , per group). Data are expressed as ; and .