Developmental and Functional Hair Cell-Like Cells Induced by Atoh1 Overexpression in the Adult Mammalian Cochlea In Vitro

Figure 2

HCLCs were successfully induced from 3 DVI in vitro, and their numbers increased with time. (a, b) HCLCs (arrowheads) at 3, 5, and 7 DVI. RFP-Atoh1- was used as controls. (c) Quantification of HCLCs per 150 μm cochlea length at 3, 5, and 7 DVI in both Atoh1+ and Atoh1- groups (). Scale bars: 15 μm in (a) and (b). Rectangular boxes show areas from which high magnification and dashed lines mark the IHC region.

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