Research Article

Developmental and Functional Hair Cell-Like Cells Induced by Atoh1 Overexpression in the Adult Mammalian Cochlea In Vitro

Figure 5

HCN currents of Atoh1-induced HCLCs and comparison of outward potassium currents of HCLCs from adult mice to normal HCs. (a) Representative HCN currents and I-V curves of HCLCs. The cell clamp was held at a holding potential of -70 mv, and the voltage steps ranged from -150 to -60 mV in 10 mV increments to evoke voltage-dependent currents. (b) Bar graph demonstrating the proportion of recorded HCN currents. (c) Representative outward K+ currents (A) recorded from P9 OHC. The I-V curves (B) show amplitudes of current density of OHCs and IHCs in P9 cochlea and HCLCs induced by Atoh1 in adult cochleae. (d) The I-V curves of OHCs and IHCs in P60 cochleae and Atoh1-induced HCLCs in adult cochleae.
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