Aβ-Induced Repressor Element 1-Silencing Transcription Factor (REST) Gene Delivery Suppresses Activation of Microglia-Like BV-2 Cells

Figure 1

Aβ_1-42 induced BV-2 cell activation. (a) Aβ_1-42 promotes BV-2 cell proliferation. The proliferation of BV-2 cells increased with the increase of Aβ_1-42 treatment time and concentration. Both 24 h and 48 h treatment of Aβ_1-42 could induce the proliferation of BV-2 cells. Only 5 μM Aβ_1-42 induced BV-2 cell proliferation significantly at the treatment time of 24 h, while both 2.5 μM and 5 μM could promote BV-2 cell proliferation at the treatment time of 48 h. (b) Aβ_1-42 induced morphological changes in BV-2 cells. Under the action of Aβ_1-42, BV-2 cells presented shortening of the processes and swelling of the soma. (c) Quantitative statistics of the increase of ameba-like cell proportion under treatment of Aβ_1-42. (d, e) Under the treatment of Aβ_1-42, MHC II protein levels were upregulated while Arg1 was downregulated with the increase of concentration of Aβ_1-42. vs. control; vs. control.

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