Research Article

Annexin A3 as a Marker Protein for Microglia in the Central Nervous System of Rats

Figure 3

Colocalization of ANXA3 and CD11b in different regions of the CNS. (a) Representative immunofluorescence images of microglia labeled by ANXA3 and CD11b in the olfactory bulb, cortex, hippocampus, thalamus, cerebellum, and spinal cord of adult rats (3 months). . (b) Images of the negative control (without primary antibody). . (c) Quantitative analysis of ANXA3+/CD11b+ microglial cells in different regions of the CNS. The ANXA3+/CD11b+ are the number of ANXA3-positive cells/the number of CD11b-positive cells in the same randomly selected field of view. The data are presented as the and were analyzed by one-way ANOVA with Tukey’s post hoc test. per group. Cells in five randomly selected fields were counted.