Annexin A3 as a Marker Protein for Microglia in the Central Nervous System of Rats

Figure 6

Spatiotemporal expression of ANXA3 protein and mRNA. (a, b) The expression levels of ANXA3 protein in rat embryos from E9.5 to E19.5 were determined by Western blotting analysis. The panel shows the protein bands corresponding to ANXA3 and tubulin. The histogram indicates the quantification of the densitometric analysis. The data are expressed as the and were analyzed by one-way ANOVA with Tukey’s post hoc test. and compared with the E9.5 group. per group. (c) Real-time PCR analysis of ANXA3 mRNA expression in rat embryos from E9.5 to E19.5. The data are expressed as the and were analyzed by one-way ANOVA with Tukey’s post hoc test. and compared with the E9.5 group. per group. (d–i) The levels of ANXA3 mRNA expression in the olfactory bulb, cortex, hippocampus, thalamus, cerebellum, and spinal cord of rats aged 1, 3, 6, 9, and 12 months were determined by qPCR analysis. The data are expressed as the and were analyzed by one-way ANOVA with Tukey’s post hoc test. , , and compared with the 1 m group. per group. 1 m: 1 month; 3 m: 3 months; 6 m: 6 months; 9 m: 9 months; 12 m: 12 months.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)