Neural Plasticity / 2021 / Article / Fig 8

Research Article

Annexin A3 as a Marker Protein for Microglia in the Central Nervous System of Rats

Figure 8

The proliferation of microglial N9 cells. (a, b) Flow cytometry analysis showing the proliferation of the Con, LV-empty, LV-NC, LV-ANXA3, and LV-shANXA3-treated N9 microglial cells. MultiCycle AV software was used to perform the fit analysis of the DNA cell cycle and to calculate the percentages of cells in each phase. The blue peak, the green arc-shaped hatched portion in the middle, and the red peak represent the G1, S, and G2 phases of the cell cycle, respectively. The data are expressed as the and were analyzed by one-way ANOVA with Tukey’s post hoc test. and compared with the LV-empty group. ## and ### compared with the LV-NC group. The data were pooled from five independent experiments. (c, d) The EdU incorporation assay showing the proliferation of the Con, LV-empty, LV-NC, LV-ANXA3, and LV-shANXA3-treated N9 microglial cells. The cell nuclei were stained with DAPI. EdU-positive cells are indicated in pink. The data are expressed as the and were analyzed by one-way ANOVA with Tukey’s post hoc test. compared with the LV-empty group. ## compared with the LV-NC group. The data were pooled from five independent experiments. . DAPI: 4,6-diamidino-2-phenylindole; EdU: 5-ethynyl-2-deoxyuridine.
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