Electroacupuncture Ameliorates Neuroinflammation-Mediated Cognitive Deficits through Inhibition of NLRP3 in Presenilin1/2 Conditional Double Knockout Mice
MCC950 does not further increase the effect of EA on inhibiting NLRP3 inflammasome and neuroinflammatory responses in the hippocampus of PS cDKO mice. (a) Representative Western blot of NLRP3, ASC, and caspase-1 in the hippocampus. (b)–(d) Quantitative analysis of NLRP3, ASC, and caspase-1 in the hippocampus were standardized based on the respective level of β-actin ( mice for each group). (e, f) Total RNA was isolated and subjected to quantitate the mRNA levels of IL-1β and IL-18 in the hippocampus by qRT-PCR ( mice for each group). The mRNA levels were standardized based on the respective level of β-actin. Values were expressed as relative changes to WT mice, which was set to 1.0. Data are the ,, vs WT mice. #,## vs cDKO mice.