Research Article

Low-Intensity Ultrasound Causes Direct Excitation of Auditory Cortical Neurons

Figure 2

Ultrasound stimulation induces inward current in cultured cortical neurons. (a) A representative confocal image shows the immunofluorescence of MAP2 (red), a marker of mature neurons, of the cultured neurons after 14 days in vitro. The nuclei were labeled by DAPI (blue). . (b) The membrane current recording of a representative neuron in response to US stimuli. The dashed lines show the stimulation of US pulses. (c, d) The mean frequency (c) and amplitude (d) of inward current of neurons with or without US stimulation. The response of HEK293 cells is compared as the control. Data are presented as the . ; ; for each group, one-way ANOVA.
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