Subarachnoid Transplant of the Human Neuronal hNT2.19 Serotonergic Cell Line Attenuates Behavioral Hypersensitivity without Affecting Motor Dysfunction after Severe Contusive Spinal Cord Injury
Figure 1
Morphology of the differentiation of the hNT2.19 and hNT2.6 cell lines in vitro. The hNT2.19 and hNT2.6 cell lines were treated for two weeks with retinoic acid (RA) and mitotic inhibitors and lifted to substrate-coated 8-well plastic tissue culture (TC) slides for differentiation and phase microscopy. As soon as 1 day of differentiation in culture under these conditions (a), hNT2.19 cells extend multipolar fibers, which are clearly visible at three days (b). The hNT2.19 cells continue to extend multiple fibers, forming a dense fiber network by 2 wks (c). The negative control hNT2.6 cells appear quite similar at 2 wks of differentiation (d). Magnification bar = 10 nm, (c); magnification bar = 20 nm, (a, b, d).
