Subarachnoid Transplant of the Human Neuronal hNT2.19 Serotonergic Cell Line Attenuates Behavioral Hypersensitivity without Affecting Motor Dysfunction after Severe Contusive Spinal Cord Injury
Figure 6
HPLC analysis: 5HT and norepinephrine content (synthesis), secretion, and release in hNT2.19 and hNT2.6 cells in vitro. The hNT2.19 (a) and the hNT2.6 (b) cell lines were differentiated, after RA and mitotic inhibitor treatment, for two weeks in 6-well substrate-coated plates before cell lysis and examination of cell content for 5HT or norepinephrine by HPLC methods. For 5HT and norepinephrine secretion (basal) and release (stimulated), sister cultures of the hNT2.19 and hNT2.6 cells were differentiated for two weeks before cells were exposed to basal (2.95 mM) or high (100 mM) concentrations of KCl for potassium (K+)-stimulated release for 5HT and norepinephrine measurement in the media. Data represent the mean + SEM from 6–18 samples from >3 independent experiments for each neurotransmitter. Only the hNT2.19 cells contain any 5HT, which is either secreted or released into the extracellular environment; the hNT2.6 cells do not secrete or release 5HT outside the cells. Neither cell line makes, secretes, or releases the neurotransmitter norepinephrine.
