Subarachnoid Transplant of the Human Neuronal hNT2.19 Serotonergic Cell Line Attenuates Behavioral Hypersensitivity without Affecting Motor Dysfunction after Severe Contusive Spinal Cord Injury

Table 2

(a) ANOVA showing thermal hyperalgesia thresholds, (b) Post hoc tests Thermal hyperalgesia (Bonferroni corrected 𝐏 values shown).

(a)

Time after contusion	Group 1: laminectomy; 𝑁=5	Group 2: contusion; 𝑁=7*	Group 3: contusion + hNT2-6; 𝑁=6*	Group 4: contusion + hNT2-19; 𝑁=7*	Group 5: laminectomy + hNT-19; 𝑁=5	Group 6: laminectomy + hNT2-6; 𝑁=7	One way ANOVA
	Mean (SEM)	Mean (SEM)	Mean (SEM)	Mean (SEM)	Mean (SEM)	Mean (SEM)	𝐹-statistic, 𝑃 value
Baseline	14.6 (0.09)	14.7 (0.14)	14.5 (0.18)	14.5 (0.10)	14.3 (0.12)	14.2 (0.17)	1.46, 𝑃=.230 (ns)
7	14.2 (0.17)^a	7.11 (0.21)^b	NA	NA	NA	NA	607.0, 𝑃<.000
14 (10)*	14.2 (0.26)^a	6.53 (0.41)^b	8.17 (0.26)^c	7.84 (0.45)^d	14.5 (0.12)^e	14.4 (0.17)^f	144.3, 𝑃<.000
21	14.0 (0.11)^a	7.63 (0.31)^b	8.46 (0.27)^c	11.4 (0.39)^d	14.7 (0.10)^e	14.5 (0.15)^f	137.3, 𝑃<.000
28	13.9 (0.13)^a	7.36 (0.18)^b	7.80 (0.39)^c	12.2 (0.26)^d	14.2 (0.11)^e	14.2 (0.18)^f	184.3, 𝑃<.000
35	14.3 (0.12)^a	7.54 (0.08)^b	8.02 (0.38)^c	12.3 (0.28)^d	14.6 (0.16)^e	14.4 (0.09)^f	232.5, 𝑃<.000
42	14.2 (0.10)^a	7.26 (0.08)^b	7.73 (0.35)^c	13.3 (0.40)^d	14.1 (0.30)^e	14.4 (0.10)^f	173.8, 𝑃<.000
49	14.5 (0.20)^a	7.56 (0.14)^b	7.89 (0.42)^c	12.8 (0.29)^d	14.4 (0.16)^e	14.6 (0.12)^f	189.1, 𝑃<.000
56	14.8 (0.11)^a	7.50 (0.12)^b	7.76 (0.49)^c	13.2 (0.49)^d	14.6 (0.10)^e	14.6 (0.18)^f	120.7, 𝑃<.000

*Time after contusion 𝑡14 is 10 days for groups 2, 3, 4 and 14 days for 1, 6, and 7.
Post hoc analyses (Bonferroni):
^aGroup 1 is significantly different than Groups 2, 3, 4 at all time points except for 𝑡42 when it is not significantly different from group 4.
^bGroup 2 is significantly different than Groups 1, 4, 5, 6 at all time points except for 𝑡14 when it is not significantly different from group 4 but from group 3.
^cGroup 3 is significantly different than Groups 1, 4, 5, 6 at all time points except for 𝑡14 when it is not significantly different from group 4 but from group 3.
^dGroup 4 is significantly different from all groups on times 21, 28, 35, 49; 𝑡14: significantly different from groups 1, 5, 6; 𝑡42: significantly different from groups 23; 𝑡56: all but 5 and 6.
^eGroup 5 is significantly different from groups 2, 3, 4 on all time points except at 𝑡42 and 𝑡56, where group 5 is significantly different only from groups 2 and 3.
^fGroup 6 is significantly different from groups 2, 3, 4 on all time points except at 𝑡42 and 𝑡56 where group 6 is significantly different from groups 2 and 3 only.

(b)

Time after surgery	Gr 1					Gr 2				Gr 3			Gr 4		Gr 5
	versus					versus				versus			versus		versus
Groups	2	3	4	5	6	3	4	5	6	4	5	6	5	6	6
TH Baseline	1.0	1.0	1.0	1.0	1.0	1.0	1.0	1.0	0.408	1.0	1.0	1.0	1.0	1.0	1.0
TH 14 (10)*	0.000	0.000	0.000	1.0	1.0	0.19	0.90	0.000	0.000	1.0	0.000	0.000	0.000	0.000	1.0
TH 21	0.000	0.000	0.000	1.0	1.0	0.486	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	1.0
TH 28	0.000	0.000	0.001	1.0	1.0	1.0	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	1.0
TH 35	0.000	0.000	0.000	1.0	1.0	1.0	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	1.0
TH 42	0.000	0.000	0.226	1.0	1.0	1.0	0.000	0.000	0.000	0.000	0.000	0.000	0.448	0.051	1.0
TH 49	0.000	0.000	0.000	1.0	1.0	1.0	0.000	0.000	0.000	0.000	0.000	0.000	0.001	0.000	1.0
TH 56	0.000	0.000	0.028	1.0	1.0	1.0	0.000	0.000	0.000	0.000	0.000	0.000	0.111	1.0	1.0