Short and Long-Term Analysis and Comparison of Neurodegeneration and Inflammatory Cell Response in the Ipsilateral and Contralateral Hemisphere of the Neonatal Mouse Brain after Hypoxia/Ischemia

Figure 6

GFAP immunostaining showing age-matched controls and effects of H/I on the hippocampus and corpus callosum. Developmental changes in astrocytes are observed in control animals (a–c), showing a progressive change from more activated astrocytes (a) to resting astrocytes at P14 (b) and P21 (c). Activated astrocytes increase in the contralateral (CL) (d–f), and ipsilateral hemisphere (IL), from 3 hours (hrs) to 100 days after hypoxia (g–t). At 3 hrs (g), astrocyte activation can be seen in the hippocampal fissure, CA and corpus callosum. At 48 hrs (i, m) and 72 hrs, (j) CA layer degeneration is observed. From 7 days (n) to 100 days (t), there is a decrease of the astrocytes activation in the corpus callosum, but in the hippocampus the reduction in astrogliosis starts at 30 days after hypoxia (p). Scale bars (low magnifications: a–j, n–p and t) = 100 μm; scale bar in all other micrographs = 20 μm. CA1: cornu ammonis 1; CC: corpus callosum; hf: hippocampal fissure.