Differential Expression of Tyrosine Hydroxylase Protein and Apoptosis-Related Genes in Differentiated and Undifferentiated SH-SY5Y Neuroblastoma Cells Treated with MPP⁺

Figure 2

Expression of tyrosine hydroxylase in undifferentiated and differentiated SH-SY5Y cells. Cells were differentiated in 10-μM retinoic acid (RA). After differentiation for 4, 7, and 10 days, tyrosine hydroxylase (TH) was detected with western blotting (a and b). The density of bands was analyzed in comparison with that of β-actin. Data are expressed as mean ± SEM (). ; . Expression of TH was visualized through immunostaining in undifferentiated cells and 4- and 7-day RA-differentiated cells (c). White arrows indicate areas of neurite outgrowth.

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(b)

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