Table 1: Inclusion and exclusion criteria for i.v. rt-PA at Thammasat University Hospital.

Inclusion criteria for i.v. rt-PA administration:
□ 1. Clinical diagnosis of acute stroke
□ 2. Well-established onset less than 4.5 hours
□ 3. No contra-indication for rt-PA administration as per exclusion criteria
□ 4. Non-contrast CT (NCCT) brain: negative for hemorrhage

Exclusion criteria for i.v. rt-PA administration:
□ 1. Only minor stroke symptoms
 -Pure sensory symptoms
 -Ataxia only
 -Motor score < 1 on initial NIHSS
□ 2. Major symptoms are recovered before initiation of i.v. rt-PA infusion.
□ 3. Any evidence of hemorrhage in the initial NCCT.
□ 4. Highly suspicious of SAH although the initial NCCT is negative.
□ 5. Evidence of pregnancy
□ 6. Laboratory values:
 -Platelet count < 100,000
 -INR > 1.7
 -Glucose < 50
 -Hb < 10
□ 7. Major surgery or serious trauma within the last 14 days
□ 8. Serious head trauma or intracranial surgery in the last 3 months
□ 9. Intracranial neoplasm, arteriovenous malformation, or aneurysm
□ 10. Active GI or GU bleeding or history of such bleeding within the last 21 days
□ 11. Recent arterial puncture at a non-compressible site (i.e., subclavian)
□ 12. Lumbar puncture within the last 7 days (including epidural/spinal anesthesia)
□ 13. Persistent hypertension
 -Unable to lower blood pressure below 185/110 within time window of treatment
 -Too aggressive treatment required to reduce blood pressure below 185/110
□ 14. History of major cerebral infarction within the past three months
□ 15. History of intracranial hemorrhage considered to put the patient at increased risk of intracranial hemorrhage
□ 16. Concurrent serious illness
□ 17. Clinical diagnosis of myocardium infarction or aortic dissection
□ 18. Clinical presentation suggestive of post-myocardial infarction pericarditis
□ 19. Seizures at onset of acute stroke
□ 20. History or clinical/lab evidence of acute drug abuse
Additional exclusion criteria for symptom onset during 3 to 4.5 hrs:
□ 1. Age > 80 years
□ 2. Taking Warfarin regardless of INR
□ 3. Initial NIHSS > 25
□ 4. Diagnosed as diabetic mellitus with previous ischemic stroke

i.v. rt-PA = intravenous recombinant tissue plasminogen activator; NCCT = noncontrast computer tomography; SAH = subarachnoid hemorrhage; NIHSS = National Institute of Health Stroke Scale; INR = international ratio; Hb = hemoglobin; GI = gastrointestinal; GU = genitourinary.