The SP/NK1R System-Mediated ROS Generation in GBM Cells through Inhibiting Glutaredoxin Protein

Figure 2

The effects of SP and aprepitant on intracellular ROS levels. U87 glioblastoma cells are exposed to the preferred concentration of SP (100 and 400 nM) alone and in combination with aprepitant (15 μM) for 24 h, and ROS formation was assessed by the DCFH-DA assay. The results demonstrate that intracellular ROS production is significantly reduced in cells treated with aprepitant with or without the pretreatment with SP.